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elisa protocol  (MultiSciences Biotech Co Ltd)
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MultiSciences Biotech Co Ltd elisa protocol
In vitro immune induction expression testing. (A) CRT in different experimental groups. Scale bar = 50 μm. (B) Columnar analysis depicting CRT induction in different experimental groups. (C) Intracellular ATP release post-administration of treatments. (D) Expression analysis of secreted HMGB-1 protein in cells after treatment administration. (E) In vitro TNF α expression profiles in different treatment groups. (F) In vitro IL-6 expression patterns in different treatment groups. (G) In vitro <t>IFN-</t> γ expression profiles in different treatment groups. (H) Analysis of the in vitro -induced maturation outcomes of DCs. α represents the combination of HPC and HPAP, l represents light and d represents dark. Data are presented as mean ± SD ( n = 3). The P values were calculated by ANOVA with Tukey's test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.
Elisa Protocol, supplied by MultiSciences Biotech Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa protocol  (Elabscience Biotechnology)
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In vitro immune induction expression testing. (A) CRT in different experimental groups. Scale bar = 50 μm. (B) Columnar analysis depicting CRT induction in different experimental groups. (C) Intracellular ATP release post-administration of treatments. (D) Expression analysis of secreted HMGB-1 protein in cells after treatment administration. (E) In vitro TNF α expression profiles in different treatment groups. (F) In vitro IL-6 expression patterns in different treatment groups. (G) In vitro <t>IFN-</t> γ expression profiles in different treatment groups. (H) Analysis of the in vitro -induced maturation outcomes of DCs. α represents the combination of HPC and HPAP, l represents light and d represents dark. Data are presented as mean ± SD ( n = 3). The P values were calculated by ANOVA with Tukey's test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.
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elisa protocol  (Arbor Assays)
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In vitro immune induction expression testing. (A) CRT in different experimental groups. Scale bar = 50 μm. (B) Columnar analysis depicting CRT induction in different experimental groups. (C) Intracellular ATP release post-administration of treatments. (D) Expression analysis of secreted HMGB-1 protein in cells after treatment administration. (E) In vitro TNF α expression profiles in different treatment groups. (F) In vitro IL-6 expression patterns in different treatment groups. (G) In vitro <t>IFN-</t> γ expression profiles in different treatment groups. (H) Analysis of the in vitro -induced maturation outcomes of DCs. α represents the combination of HPC and HPAP, l represents light and d represents dark. Data are presented as mean ± SD ( n = 3). The P values were calculated by ANOVA with Tukey's test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.
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capture elisa protocol  (bioMerieux gmbh)
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In vitro immune induction expression testing. (A) CRT in different experimental groups. Scale bar = 50 μm. (B) Columnar analysis depicting CRT induction in different experimental groups. (C) Intracellular ATP release post-administration of treatments. (D) Expression analysis of secreted HMGB-1 protein in cells after treatment administration. (E) In vitro TNF α expression profiles in different treatment groups. (F) In vitro IL-6 expression patterns in different treatment groups. (G) In vitro <t>IFN-</t> γ expression profiles in different treatment groups. (H) Analysis of the in vitro -induced maturation outcomes of DCs. α represents the combination of HPC and HPAP, l represents light and d represents dark. Data are presented as mean ± SD ( n = 3). The P values were calculated by ANOVA with Tukey's test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.
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prostaglandin e2 elisa kit—monoclonal protocol cayman #514010  (Cayman Chemical)
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In vitro immune induction expression testing. (A) CRT in different experimental groups. Scale bar = 50 μm. (B) Columnar analysis depicting CRT induction in different experimental groups. (C) Intracellular ATP release post-administration of treatments. (D) Expression analysis of secreted HMGB-1 protein in cells after treatment administration. (E) In vitro TNF α expression profiles in different treatment groups. (F) In vitro IL-6 expression patterns in different treatment groups. (G) In vitro <t>IFN-</t> γ expression profiles in different treatment groups. (H) Analysis of the in vitro -induced maturation outcomes of DCs. α represents the combination of HPC and HPAP, l represents light and d represents dark. Data are presented as mean ± SD ( n = 3). The P values were calculated by ANOVA with Tukey's test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.
Prostaglandin E2 Elisa Kit—Monoclonal Protocol Cayman #514010, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prostaglandin e 2 elisa kit—monoclonal protocol  (Cayman Chemical)
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In vitro immune induction expression testing. (A) CRT in different experimental groups. Scale bar = 50 μm. (B) Columnar analysis depicting CRT induction in different experimental groups. (C) Intracellular ATP release post-administration of treatments. (D) Expression analysis of secreted HMGB-1 protein in cells after treatment administration. (E) In vitro TNF α expression profiles in different treatment groups. (F) In vitro IL-6 expression patterns in different treatment groups. (G) In vitro <t>IFN-</t> γ expression profiles in different treatment groups. (H) Analysis of the in vitro -induced maturation outcomes of DCs. α represents the combination of HPC and HPAP, l represents light and d represents dark. Data are presented as mean ± SD ( n = 3). The P values were calculated by ANOVA with Tukey's test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.
Prostaglandin E 2 Elisa Kit—Monoclonal Protocol, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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standardization of elisa protocols for serosurveys of the sars-cov-2 pandemic using clinical and at-home blood sampling  (Sadtler Research Laboratories)
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Sadtler Research Laboratories standardization of elisa protocols for serosurveys of the sars-cov-2 pandemic using clinical and at-home blood sampling
In vitro immune induction expression testing. (A) CRT in different experimental groups. Scale bar = 50 μm. (B) Columnar analysis depicting CRT induction in different experimental groups. (C) Intracellular ATP release post-administration of treatments. (D) Expression analysis of secreted HMGB-1 protein in cells after treatment administration. (E) In vitro TNF α expression profiles in different treatment groups. (F) In vitro IL-6 expression patterns in different treatment groups. (G) In vitro <t>IFN-</t> γ expression profiles in different treatment groups. (H) Analysis of the in vitro -induced maturation outcomes of DCs. α represents the combination of HPC and HPAP, l represents light and d represents dark. Data are presented as mean ± SD ( n = 3). The P values were calculated by ANOVA with Tukey's test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.
Standardization Of Elisa Protocols For Serosurveys Of The Sars Cov 2 Pandemic Using Clinical And At Home Blood Sampling, supplied by Sadtler Research Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa kit protocol  (Jingmei Biotech Co Ltd)
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In vitro immune induction expression testing. (A) CRT in different experimental groups. Scale bar = 50 μm. (B) Columnar analysis depicting CRT induction in different experimental groups. (C) Intracellular ATP release post-administration of treatments. (D) Expression analysis of secreted HMGB-1 protein in cells after treatment administration. (E) In vitro TNF α expression profiles in different treatment groups. (F) In vitro IL-6 expression patterns in different treatment groups. (G) In vitro <t>IFN-</t> γ expression profiles in different treatment groups. (H) Analysis of the in vitro -induced maturation outcomes of DCs. α represents the combination of HPC and HPAP, l represents light and d represents dark. Data are presented as mean ± SD ( n = 3). The P values were calculated by ANOVA with Tukey's test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.
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elisa-based protocol tecan tech spark 10m  (Tecan Systems)
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In vitro immune induction expression testing. (A) CRT in different experimental groups. Scale bar = 50 μm. (B) Columnar analysis depicting CRT induction in different experimental groups. (C) Intracellular ATP release post-administration of treatments. (D) Expression analysis of secreted HMGB-1 protein in cells after treatment administration. (E) In vitro TNF α expression profiles in different treatment groups. (F) In vitro IL-6 expression patterns in different treatment groups. (G) In vitro <t>IFN-</t> γ expression profiles in different treatment groups. (H) Analysis of the in vitro -induced maturation outcomes of DCs. α represents the combination of HPC and HPAP, l represents light and d represents dark. Data are presented as mean ± SD ( n = 3). The P values were calculated by ANOVA with Tukey's test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.
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elisa protocols/kits  (IDEXX)
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In vitro immune induction expression testing. (A) CRT in different experimental groups. Scale bar = 50 μm. (B) Columnar analysis depicting CRT induction in different experimental groups. (C) Intracellular ATP release post-administration of treatments. (D) Expression analysis of secreted HMGB-1 protein in cells after treatment administration. (E) In vitro TNF α expression profiles in different treatment groups. (F) In vitro IL-6 expression patterns in different treatment groups. (G) In vitro <t>IFN-</t> γ expression profiles in different treatment groups. (H) Analysis of the in vitro -induced maturation outcomes of DCs. α represents the combination of HPC and HPAP, l represents light and d represents dark. Data are presented as mean ± SD ( n = 3). The P values were calculated by ANOVA with Tukey's test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.
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In vitro immune induction expression testing. (A) CRT in different experimental groups. Scale bar = 50 μm. (B) Columnar analysis depicting CRT induction in different experimental groups. (C) Intracellular ATP release post-administration of treatments. (D) Expression analysis of secreted HMGB-1 protein in cells after treatment administration. (E) In vitro TNF α expression profiles in different treatment groups. (F) In vitro IL-6 expression patterns in different treatment groups. (G) In vitro IFN- γ expression profiles in different treatment groups. (H) Analysis of the in vitro -induced maturation outcomes of DCs. α represents the combination of HPC and HPAP, l represents light and d represents dark. Data are presented as mean ± SD ( n = 3). The P values were calculated by ANOVA with Tukey's test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.

Journal: Acta Pharmaceutica Sinica. B

Article Title: Polymer-assisted PD-L1 degradation and targeted photodynamic therapy synergize to suppress immunodeficient tumors

doi: 10.1016/j.apsb.2025.05.022

Figure Lengend Snippet: In vitro immune induction expression testing. (A) CRT in different experimental groups. Scale bar = 50 μm. (B) Columnar analysis depicting CRT induction in different experimental groups. (C) Intracellular ATP release post-administration of treatments. (D) Expression analysis of secreted HMGB-1 protein in cells after treatment administration. (E) In vitro TNF α expression profiles in different treatment groups. (F) In vitro IL-6 expression patterns in different treatment groups. (G) In vitro IFN- γ expression profiles in different treatment groups. (H) Analysis of the in vitro -induced maturation outcomes of DCs. α represents the combination of HPC and HPAP, l represents light and d represents dark. Data are presented as mean ± SD ( n = 3). The P values were calculated by ANOVA with Tukey's test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.

Article Snippet: After a 12 h incubation period in standard culture media, the cells were subjected to various treatment conditions, including PBS, HPAP, HPC-dark, HPC-light, (HPC + HPAP)-dark, and (HPC + HPAP)-light groups, for an additional incubation period of 24 h. Subsequent to these treatments, for the intricacies of immune response analysis, culture media supernatants were harvested and assayed for the presence of cytokines TNF α , IL-6, and IFN- γ employing an ELISA protocol detailed by the manufacturer (MultiSciences Biotech Co., Ltd.

Techniques: In Vitro, Expressing